The control of surface properties and spatial presentation of functional molecules within a microfluidic channel is important for the development of diagnostic assays, microreactors, and for performing fundamental studies of cell biology and fluid mechanics. Here, we present soft lithographic methods to create robust microchannels with patterned microstructures inside the channel. The patterned regions were protected from oxygen plasma by controlling the dimensions of the poly(dimethylsiloxane) (PDMS) mold as well as the sequence of fabrication steps. The approach was used to pattern a non-biofouling polyethylene glycol (PEG)-based copolymer or the polysaccharide hyaluronic acid (HA) within microfluidic channels. These non-biofouling patterns were then used to fabricate arrays of fibronectin (FN) and bovine serum albumin (BSA) as well as mammalian cells.

Keywords: Microfluidics,Soft lithography,Patterning,Protein,Cell,마이크로유체역학,소프트 리소그라피,패터닝,단백질,세포