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Applied Science and Convergence Technology 2013; 22(2): 55-65

Published online March 1, 2013

Copyright © The Korean Vacuum Society.

Sterilization of Neurospora Crassa by Noncontacted Low Temperature Atmospheric Pressure Surface Discharged Plasma with Dielectric Barrier Structure

Young Hyo Ryu , Han Sup Uhm , Gyung Soon Park , Eun Ha Choi

Abstract

Sterilization of Neurospora crassa has been investigated in this research by using a surface air plasma with dielectric barrier discharged (DBD) structure under atmospheric pressure. The sinusoidal alternating current has been used in this experiment with discharge voltage of 1.4∼2.3 kV. The phase difference between the voltage and current signals are found to be almost 80 degree due to the capacitive property of dielectric barrier. Temperature on the biomaterials has been minimized by radiating the heat with the air cooling system. It is noted that the substrate temperature remains under 37 degree for plasma exposure time of 10 minutes with operation of cooler system. It is found that the ozone, O3, has been measured to be about 25∼30 ppm within 1 cm region and to be about 5 ppm at the 150 cm downstream region away from the suface plasma. It is also noted that the nitric oxide, NO, and nitric dioxide, NO2, are not nearly detected. Germination rate and mitochodrial activity of Neurospora crassa immersed in the deionized water have been found to be drastically decreased as the plasma treatment time and its electrical power are increased in this experiment. Here, the mitochondrial activity has been analyzed by MTT (3-(4,5-dimethy lthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. However, sterilization of Neurospora crassa immersed in the Vogel"s minimal media has been found to be low by plasma treatment, which is caused by surrounding background solution. This research shows the sterilization possibility of Neurospora crassa by using the noncontated surface DBD plasma, which is different from the plasma jet. This is mainly attibuted to the reactive species generated by the surface plasma, since they play a major role for inhibition of micobes such as Neurospora crassa.

Keywords: 유전체장벽 방전구조,저온 대기압 면방전,플라즈마,빵 곰팡이,살균,활성종,Dielectric barrier discharge,Low temperature atmospheric pressure surface discharged,Plasma,Neurospora crassa,Sterilization,Reactive species

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