Applied Science and Convergence Technology 2023; 32(6): 134-140
Published online November 30, 2023
https://doi.org/10.5757/ASCT.2023.32.6.134
Copyright © The Korean Vacuum Society.
Rochani Manishikaa , b , † , Sung Eun Seoa , c , † , Kyung Ho Kima , d , Moo-Seung Leee , f , * , and Oh Seok Kwona , g , h , *
aInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
bDepartment of Bio-molecular Science, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
cDepartment of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea
dImmunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
eEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
fBiomolecular Science, Korea University of Science and Technology, Daejeon 34113, Republic of Korea
gSKKU Advanced Institute of Nanotechnology (SAINT), Department of Nano Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
hDepartment of Nano Science and Technology, Sungkyunkwan University, Suwon 16419, Republic of Korea
Correspondence to:msl031000@kribb.re.kr, oskwon79@skku.edu
†These authors contributed equally to this work as first author.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, distribution and reproduction in any medium without alteration, provided that the original work is properly cited.
The amplification of genes [deoxyribose nucleic acid (DNA) and ribose nucleic acid] is a vital molecular tool not only in elementary research but also in application-oriented fields, such as industrial quality control, infectious disease diagnosis, clinical medicine development, and gene cloning. Gene amplification is directed by a photonic thermocycler, which is programmed for the alteration of the reaction temperature every few minutes to allow DNA denaturation and synthesis. Photonic polymerase chain reaction (PCR) represents a novel solution for the rapid amplification of genetic materials to achieve the goals of point-of-care diagnosis in a pandemic situation. Some nanoscale materials are able to emit thermal energy after the absorption of radiation with a wide range of frequency. This energy conversion process has been characterized by the surface plasmatic resonance of photothermal nanomaterials highly related to photonic technology. Many photonic PCR systems related to photothermal activity have been proposed while minimizing the thermal cycle time with photonic PCR techniques. The thermal cycle time of the photonic PCR is faster than conventional PCR applications, and this new technique is thought to have potential for the application to clinical and environmental diagnostics, which is the field with a need for rapid diagnosis.
Keywords: Photonic polymerase chain reaction, Nucleic acid amplification, Photothermal activity, Plasma heating